| 產(chǎn)品編號 | bs-10683R-APC |
| 英文名稱1 | Rabbit Anti-phospho-STK25 (Thr174)/APC Conjugated antibody |
| 中文名稱 | APC標記的絲氨酸/蘇氨酸激酶25抗體 |
| 別 名 | STK25 (phospho T174); p-STK25 (phospho T174); Serine/threonine kinase 25; Serine/threonine kinase 25 (STE20 homolog, yeast); Serine/threonine kinase 25 (STE20 homolog, yeast), isoform CRA_a; Serine/threonine kinase 25 (yeast); Serine/threonine protein kinase 25; Serine/threonine-protein kinase 25; SOK-1; SOK1; Ste20 like kinase; Ste20 yeast homolog; Ste20-like; STE20-like kinase; Ste20/oxidant stress response kinase 1; Sterile 20 (oxidant stress response kinase 1); Sterile 20/oxidant stress-response kinase 1; STK25; STK25_HUMAN; Yeast Sps1/Ste20 related kinase 1; YSK1; C86992. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 產(chǎn)品類型 | 磷酸化抗體 |
| 研究領域 | 細胞生物 神經(jīng)生物學 信號轉導 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human, Mouse, Rat, Cow, Horse, Sheep, ) |
| 產(chǎn)品應用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 48kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthesised phosphopeptide derived from human STK25 around the phosphorylation site of Thr174 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: Oxidant stress-activated serine/threonine kinase that may play a role in the response to environmental stress. Targets to the Golgi apparatus where it appears to regulate protein transport events, cell adhesion, and polarity complexes important for cell migration. Several mammalian kinases have been identified which exhibit sequence similarity to the Saccharomyces cerevisiae serine/threonine kinase STE20. STE20 is involved in relaying signals from G-protein coupled receptors, and it lies upstream of a MAP kinase kinase kinase. Mammalian STE20-like kinases include YSK1, KHS, GLK, NIK, HPK1, Krs-1, Krs-2 and GC kinase. YSK1 (yeast SPS/STE20-related kinase 1) is expressed in a wide variety of cell types and tissues and has been shown to have kinase activity. Unlike many of the other STE20-like kinases, however, overexpression of YSK1 does not lead to activation of the SAPK/JNK pathway. Function: Oxidant stress-activated serine/threonine kinase that may play a role in the response to environmental stress. Targets to the Golgi apparatus where it appears to regulate protein transport events, cell adhesion, and polarity complexes important for cell migration. Subunit: Homodimer. Subcellular Location: Cytoplasm. Golgi apparatus. Localizes to the Golgi apparatus. Tissue Specificity: Ubiquitously expressed. Highest levels are found in testis, large intestine, brain and stomach followed by heart and lung. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. Contains 1 protein kinase domain. Database links: Entrez Gene: 10494 Human Entrez Gene: 51765 Human Entrez Gene: 8428 Human Omim: 300547 Human Omim: 602255 Human Omim: 604984 Human SwissProt: O00506 Human SwissProt: Q9P289 Human SwissProt: Q9Y6E0 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
