| 產品編號 | bs-13506R-PE-Cy5.5 |
| 英文名稱1 | Rabbit Anti-GPR77/PE-Cy5.5 Conjugated antibody |
| 中文名稱 | PE-Cy5.5標記的G蛋白偶聯(lián)受體77抗體 |
| 別 名 | C5a anaphylatoxin chemotactic receptor C5L2; C5ARL_HUMAN; C5L2; E030029A11Rik; G protein coupled receptor 77; G protein coupled receptor C5L2; G-protein coupled receptor 77; GPF77; GPR77; GPCR GPR77. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 細胞生物 信號轉導 G蛋白偶聯(lián)受體 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human, ) |
| 產品應用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 36kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GPF77 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產品介紹 |
background: Anaphylatoxin, a chemotactic and inflammatory peptide, is a receptor for C5a anaphylatoxin chemotactic receptor (C5L2), also designated G protein-coupled receptor 77. C5L2 is an integral membrane protein that belongs to the Gprotein-coupled receptor 1 family. C5L2 weakly couples to Gi-mediated signaling pathways and is widely expressed in the hippocampus, hypothalamus, pons, frontal cortex and liver. It is coexpressed with the C5a receptor (C5AR) on polymorphonuclear neutrophils and may modulate the activity of C5AR. The function of the C5a receptors, particularly that of C5L2, is still unclear. Research suggests that C5L2 may be required for the in vitro release of high mobility group box 1 protein (HMGB1). Function: Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a, C4a and C3a and their desarginated derivatives. Couples weakly to G(i)-mediated signaling pathways. Subunit: Interacts with C3 (the anaphylatoxin peptide C3a and the adipogenic hormone ASP); the interaction occurs with higher affinity for ASP, enhancing the phosphorylation and activation of GPR77, recruitment of ARRB2 to the cell surface and endocytosis of GRP77. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Frontal cortex, hippocampus, hypothalamus, pons and liver. Similarity: Belongs to the G-protein coupled receptor 1 family. Database links: UniProtKB/Swiss-Prot: Q9P296.1 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
