| 產(chǎn)品編號(hào) | bsm-52079R |
| 英文名稱 | FOXP3 Recombinant Rabbit mAb |
| 中文名稱 | 叉頭蛋白P3重組兔單抗 |
| 別 名 | Forkhead box protein P3; forkhead box P3; Scurfin; Forkhead box protein P3, C-terminally processed; Forkhead box protein P3 41 kDa form; JM2; AIID; IPEX; PIDX; XPID; DIETER; FOXP3_HUMAN; |
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Specific References (3) | bsm-52079R has been referenced in 3 publications.
[IF=5.722] Judith Heiss. et al. Targeted Interleukin-9 delivery in pulmonary hypertension: Comparison of immunocytokine formats and effector cell study. EUR J CLIN INVEST. 2022 Nov;:e13907 IF ; Mouse.
[IF=5.085] Wang Ning. et al. CD226 Attenuates Treg Proliferation via Akt and Erk Signaling in an EAE Model. Front Immunol. 2020 Aug;11:1883 IHC ; Mouse.
[IF=2.885] Sun, Xinxin. et al. 0.1% Nano-silver mediates PD-1/PD-L1 pathway and alleviates chronic apical periodontitis in rats. ODONTOLOGY. 2022 Sep;:1-11 IHC ; Rat.
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| 研究領(lǐng)域 | 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號(hào) | 12B1 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,Flow-Cyt=2ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 47 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human FOXP3 protein, around C-terminal 150aa |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]. Function: Probable transcription factor. Plays a critical role in the control of immune response. Subunit: Interacts with IKZF3. Subcellular Location: Nucleus (Potential). Post-translational modifications: Acetylation on lysine residues stabilizes FOXP3 and promotes differentiation of T-cells into induced regulatory T-cells (iTregs) associated with suppressive functions. Deacetylated by SIRT1. DISEASE: Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy. Similarity: Contains 1 C2H2-type zinc finger. Contains 1 fork-head DNA-binding domain. SWISS: Q9BZS1 Gene ID: 50943 Database links: Entrez Gene: 50943 Human Entrez Gene: 20371 Mouse Omim: 300292 Human SwissProt: Q9BZS1 Human SwissProt: Q99JB6 Mouse Unigene: 247700 Human Unigene: 182291 Mouse |
| 產(chǎn)品圖片 |
Sample:
293T (Mouse) Lysate at 40 ug
Primary: Anti- FOXP3 (bsm-52079R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 47 kD
Blank control:MCF7.
Primary Antibody (green line): Rabbit Anti-FOXP3 antibody (bsm-52079R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
Blank control:MCF-7.
Primary Antibody (green line): Rabbit Anti-FOXP3 antibody (bsm-52079R)
Dilution: 1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature
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