| 產(chǎn)品編號 | bs-6841R-BF488 |
| 英文名稱1 | Rabbit Anti-IL-4I1/BF488 Conjugated antibody |
| 中文名稱 | BF488標(biāo)記的白細(xì)胞介素4誘導(dǎo)蛋白1抗體 |
| 別 名 | IL4I1; hFIG1; IL4 induced protein 1; IL-4 induced protein 1; Interleukin 4 induced protein 1; LAAO; FIG 1; Fig 1 protein; FIG1; Fig1 protein; hFIG 1; hFIG1; IL4-induced protein 1; Interleukin 4 induced 1; Interleukin four induced 1; Interleukin-4-induced protein 1; L amino acid oxidase; L-amino-acid oxidase; LAAO; LAO; Protein Fig 1; Protein Fig-1; Protein Fig1; OXLA_HUMAN. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 細(xì)胞生物 細(xì)胞凋亡 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse, (predicted: Human, Rat, Dog, Pig, Sheep, ) |
| 產(chǎn)品應(yīng)用 | Flow-Cyt=2ug/Test IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 61kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human IL-4I1 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: This gene encodes a protein with limited similarity to L-amino acid oxidase which contains the conserved amino acids thought to be involved in catalysis and binding of flavin adenine dinucleotide (FAD) cofactor. The expression of this gene can be induced by interleukin 4 in B cells, however, expression of transcripts containing the first two exons of the upstream gene is found in other cell types. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]. Function: Lysosomal L-amino-acid oxidase with highest specific activity with phenylalanine. May play a role in lysosomal antigen processing and presentation (By similarity). Subcellular Location: Isoform 1: Lysosome (By similarity). Isoform 2: Lysosome (By similarity). Tissue Specificity: Isoform 1 is primarily found in immune tissues. Similarity: Belongs to the flavin monoamine oxidase family. FIG1 subfamily. Database links: Entrez Gene: 259307 Human Entrez Gene: 14204 Mouse Omim: 609742 Human SwissProt: Q1WMJ3 Human SwissProt: Q96RQ9 Human SwissProt: O09046 Mouse Unigene: 27567 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品圖片 |
Blank control: Mouse spleen.
Primary Antibody (green line): Rabbit Anti-IL-4I1 antibody (bs-6841R-A488)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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