| 產(chǎn)品編號(hào) | bs-0541R-BF647 |
| 英文名稱1 | Rabbit Anti-CAMK2A + CAMK2B + CAMK2D/BF647 Conjugated antibody |
| 中文名稱 | BF647標(biāo)記的鈣/鈣調(diào)素依賴蛋白激酶2b/2γ抗體 |
| 別 名 | CaMK2 beta+gamma+delta; CaMK2 beta; CaMK2 gamma; CaMK2 delta; CAMK2G; Calcium/calmodulin dependent protein kinase (CaM kinase) II beta; Calcium/calmodulin dependent protein kinase II beta; Calcium/calmodulin dependent protein kinase IIB; Calcium/calmodulin dependent protein kinase type II beta chain; CAM 2; CaM kinase II beta chain; CaM kinase II beta subunit; CAM2; CAMK 2; CAMK 2B; CaMK II beta subunit; CaMK II subunit beta; CAMK2; CaMK2 beta; CAMK2B; CAMKB; CaMKII beta subunit; CaMKIIB; MGC29528; Proline rich calmodulin dependent protein kinase; CaM kinase II; Calcium/calmodulin-dependent protein; CaM kinase II gamma subunit; CaM-kinase II gamma chain; CaM kinase II delta subunit; CaM-kinase II delta chain; CAM2; CAMK; CAMK-II; CAMK2D; CAMK2G; CAMK2N2; CAMKD; CAMKG; KCC2B; kinase CaMK2-beta. |
| 規(guī)格價(jià)格 | 100ul/2980元 購買 大包裝/詢價(jià) |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 腫瘤 神經(jīng)生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞周期蛋白 激酶和磷酸酶 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Mouse, Rat, |
| 產(chǎn)品應(yīng)用 | Flow-Cyt=2ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 53-73kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CaMK2 beta/ gamma/ delta |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a beta chain. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. Eight transcript variants encoding eight distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]. Function: Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplamic reticulum Ca(2+) transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. Participates in the modulation of skeletal muscle function in response to exercise. In slow-twitch muscles, is involved in regulation of sarcoplamic reticulum (SR) Ca(2+) transport and in fast-twitch muscle participates in the control of Ca(2+) release from the SR through phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2. Subunit: CAMK2 is composed of 4 different chains: alpha (CAMK2A), beta (CAMK2B), gamma (CAMK2G), and delta (CAMK2D). The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 12 subunits with two hexameric rings stacked one on top of the other. Interacts with SYNGAP1 and CAMK2N2 (By similarity). Interacts with MPDZ. Subcellular Location: Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, centrosome. Sarcoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Note=In slow-twitch muscle, evenly distributed between longitudinal SR and junctional SR. Tissue Specificity: Widely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain. Expressed in skeletal muscle. Post-translational modifications: Autophosphorylation of Thr-287 following activation by Ca(2+)/calmodulin. Phosphorylation of Thr-287 locks the kinase into an activated state. Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. Contains 1 protein kinase domain. Database links: Entrez Gene: 815 Human Entrez Gene: 816 Human Entrez Gene: 817 Human Entrez Gene: 108058 Mouse Entrez Gene: 12322 Mouse Entrez Gene: 12323 Mouse Omim: 114078 Human Omim: 607707 Human Omim: 607708 Human SwissProt: Q13554 Human SwissProt: Q13557 Human SwissProt: Q9UQM7 Human SwissProt: P11798 Mouse SwissProt: P28652 Mouse SwissProt: Q6PHZ2 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. CaMK2b也是絲/蘇氨基酸激酶的一種，同屬于Ca++/鈣調(diào)素依賴性蛋白質(zhì)激酶家族成員。鈣／鈣調(diào)素依賴性蛋白激酶Ⅱb（CaMKⅡb）廣泛分布于中樞神經(jīng)系統(tǒng)內(nèi)，參與調(diào)節(jié)神經(jīng)遞質(zhì)的合成釋放、信號(hào)轉(zhuǎn)導(dǎo)和突觸可塑性的形成以及磷酸化蛋白 |
| 產(chǎn)品圖片 |
Blank control:MCF.
Primary Antibody (green line): Rabbit Anti-CAMK2A + CAMK2B + CAMK2D antibody (bs-0541R-BF647)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1%PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.
|
