| 產(chǎn)品編號 | bsm-61027R |
| 英文名稱 | TGF beta 1 Recombinant Rabbit mAb |
| 中文名稱 | 轉(zhuǎn)化生長因子β1重組兔單抗 |
| 別 名 | CED; DPD1; LAP; Latency-associated peptide; TGFβ-1; TGFβ1; TGF beta; TGF-beta 1; TGF beta 1 protein; TGF-beta 1 protein; TGF-beta-1; TGF-beta-5; TGF-beta1; TGFB; Tgfb-1; tgfb1; TGFB1_HUMAN; TGFbeta; TGFbeta1; Transforming Growth Factor b1; Transforming Growth Factor beta 1; Transforming growth factor beta 1a; transforming growth factor beta-1; transforming growth factor, beta 1 |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 12B9 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 44 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞外基質(zhì) 分泌型蛋白 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human TGF beta 1: 300-335 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGFB family members. This encoded protein regulates cell proliferation, differentiation and growth, and can modulate expression and activation of other growth factors including interferon gamma and tumor necrosis factor alpha. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease. [provided by RefSeq, Aug 2016] Function: Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Subunit: Homodimer; disulfide-linked, or heterodimer with TGFB2. Secreted and stored as a biologically inactive form in the extracellular matrix in a 290 kDa complex (large latent TGF-beta1 complex) containing the TGFB1 homodimer, the latency-associated peptide (LAP), and the latent TGFB1 binding protein-1 (LTBP1). The complex without LTBP1 is known as the'small latent TGF-beta1 complex'. Dissociation of the TGFB1 from LAP is required for growth factor activation and biological activity. Release of the large latent TGF-beta1 complex from the extracellular matrix is carried out by the matrix metalloproteinase MMP3 (By similarity). May interact with THSD4; this interaction may lead to sequestration by FBN1 microfibril assembly and attenuation of TGFB signaling. Interacts with the serine proteases, HTRA1 and HTRA3: the interaction with either inhibits TGFB1-mediated signaling. The HTRA protease activity is required for this inhibition (By similarity). Interacts with CD109, DPT and ASPN. Subcellular Location: Secreted, extracellular space, extracellular matrix. Tissue Specificity: Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage. Post-translational modifications: Glycosylated. The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive. DISEASE: Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision. Similarity: Belongs to the TGF-beta family. SWISS: P01137 Gene ID: 7040 Database links: Entrez Gene: 7040 Human Entrez Gene: 21803 Mouse Omim: 190180 Human SwissProt: P01137 Human SwissProt: P04202 Mouse Unigene: 645227 Human 生長因子和激素（ Growth Factor and Hormones） TGF是一種多效生長因子，對上皮細(xì)胞增值有潛在抑制作用，可抑制腫瘤生長用于許多惡性腫瘤如:胃癌、肺癌、膀胱癌、腎癌、前列腺癌、結(jié)腸癌等多種惡性腫瘤的研究。 TGFβ超級家族由為數(shù)眾多的生長的分化因子組成,包括轉(zhuǎn)移生長因子β1，2和3(TGF β1，TGF β2，TGF β3)；胎盤生長因子(PTGF-β)；生長/分化因子(GDFs)；繆氏抑制物(MIS)；骨形態(tài)形成蛋白(BMPs)；交織細(xì)胞元神經(jīng)生長因子(GDNF);抑制素和活化素(α, β-A,和β-C)，Lefty和Nodal。 TGF超級家族成員參與胚胎發(fā)育和成體組織體內(nèi)平衡。TGF-β1抗體與TGF-β2和TGF-β3沒有交叉反應(yīng)。與豚鼠有大部分交叉. |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with TGF beta 1 monoclonal antibody, unconjugated (bsm-61027R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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