| 產(chǎn)品編號 | bsm-60359M |
| 英文名稱 | Annexin IV Recombinant Mouse mAb |
| 中文名稱 | 膜粘連蛋白A4重組鼠單抗 |
| 別 名 | 35 beta calcimedin; Carbohydrate binding protein P33/P41; DKFZp686H02120; Endonexin I; Lipocortin IV; P32.5; Annexin A4; Annexin IV placental anticoagulant protein II; ANX 4; ANX A4; ANX4; ANXA 4; LipocortinIV; P33/41; Placental anticoagulant protein II a |
| 研究領(lǐng)域 | 心血管 |
| 抗體來源 | Mouse |
| 克隆類型 | Recombinant |
| 克 隆 號 | D4C7 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:200-1000,IHC-P=1:20-100,IHC-F=1:400-800,IF=1:20-50,ICC/IF=1:20-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 35 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Annexin IV |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The Annexins are a family of structurally similar proteins. Annexins bind to phospholipids and may be involved in regulation of membrane transport, membrane channel activity, and interaction of the cell membrane with the extracellular matrix. Annexin A4 (ANXA4) belongs to the annexin family of calcium dependent phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane related events along exocytotic and endocytotic pathways. ANXA4 has 45 to 59% identity with other members of its family and shares a similar size and exon intron organization. Isolated from human placenta, ANXA4 encodes a protein that has possible interactions with ATP, and has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. ANXA4 is almost exclusively expressed in epithelial cells. Function: Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. Similarity: Belongs to the annexin family. Contains 4 annexin repeats. SWISS: P09525 Gene ID: 307 Database links: Entrez Gene: 307 Human Entrez Gene: 11746 Mouse Omim: 106491 Human SwissProt: P09525 Human SwissProt: P97429 Mouse Unigene: 422986 Human Unigene: 259702 Mouse Unigene: 19270 Rat |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition:4°C overnight
Lysate: HeLa, HepG2, SW480, SH-SY5Y
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 32 kDa
Observed MW: 32 kDa
Tissue: Human placenta
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin
Comment: Color brown is the positive signal for bsm-60359M
Cell line: HeLa
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for bsm-60359M
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
