| 產品編號 | bs-11548R |
| 英文名稱 | Rabbit Anti-Phospho-Dab1 (Tyr198) antibody |
| 中文名稱 | 磷酸化磷脂蛋白受體Dab1抗體 |
| 別 名 | Dab1 (phospho Y198); Dab1 (Phospho-Tyr198) ; Dab 1; Dab1; Dab-1; DAB1_HUMAN; Disabled homolog 1; Disabled homolog 1 Drosophila; Scm; Scr; Scrambler; Yot; Yotari. |
| 產品類型 | 磷酸化抗體 |
| 研究領域 | 細胞生物 神經生物學 信號轉導 細胞膜受體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Rat (predicted: Mouse,Rabbit,Pig,Cow,Chicken,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 65kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthesised phosphopeptide derived from human Dab1 around the phosphorylation site of Tyr198: V(p-Y)QY |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The laminar organization of multiple neuronal types in the cerebral cortex is required for normal cognitive function. In mice, the disabled-1 gene plays a central role in brain development, directing the migration of cortical neurons past previously formed neurons to reach their proper layer. This gene is similar to disabled-1, and the protein encoded by this gene is thought to be a signal transducer that interacts with protein kinase pathways to regulate neuronal positioning in the developing brain. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined. [provided by RefSeq, Jul 2008] Function: Adapter molecule functioning in neural development. May regulate SIAH1 activity. Subunit: Associates with the SH2 domains of SRC, FYN and ABL. Interacts with DAB2IP and SIAH1. Interacts with LRP1. Post-translational modifications: Phosphorylated on Tyr-198 and Tyr-220 upon reelin induction in embryonic neurons. Also phosphorylated on Ser-524 independently of reelin signaling. Similarity: Contains 1 PID domain. SWISS: O75553 Gene ID: 1600 Database links: Entrez Gene: 1600 Human Entrez Gene: 13131 Mouse Entrez Gene: 374083 Chicken Omim: 603448 Human SwissProt: O75553 Human SwissProt: P97318 Mouse Unigene: 477370 Human Unigene: 289682 Mouse Unigene: 206534 Rat
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| 產品圖片 |
Sample:
Lane 1: Human HeLa cell lysates
Lane 2: Human HepG2 cell lysates
Lane 3: Human MCF-7 cell lysates
Lane 4: Human SH-SY5Y cell lysates
Primary: Anti-Phospho-Dab1 (Tyr198) (bs-11548R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kDa
Observed band size: 68 kDa
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Phospho-Dab1 (Tyr198) Polyclonal Antibody, Unconjugated(bs-11548R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
