| 產(chǎn)品編號 | bs-5984R |
| 英文名稱 | Rabbit Anti-ABI2 antibody |
| 中文名稱 | 腫瘤抑制基因ABI2/精氨酸酪氨酸蛋白激酶結(jié)合蛋白抗體 |
| 別 名 | Abelson interactor 2; ABI 2; ABI2B; Abl binding protein 3; Abl interacting protein 1 (SH3 containing protein); Abl interactor 2; Abl interactor protein 2b; AblBP3; AIP 1; Arg binding protein 1; Arg protein tyrosine kinase binding protein; ArgBP1; ARGBPIA; ArgBPIB; SSH3BP2; ABI2_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse,Rat (predicted: Human,Rabbit,Pig,Sheep,Chicken,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 56kDa |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ABI2: 421-513/513 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
ABI2 (Abelson interactor 2) belongs to the ABI family. The Abelson interactors are among a group of proteins that interact with Abl tyrosine kinase, a non-receptor tyrosine kinase whose activation results in the regulation of cytoskeleton function, cell growth and apoptosis in response to a variety of biological stimuli.
Function: May act in regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Regulates ABL1/c-Abl-mediated phosphorylation of MENA. [SUBUNIT] Component of the WAVE1 complex composed of ABI2, CYFIP1 or CYFIP2, BRK1, NCKAP1 and WASF1/WAVE1. Within the complex, a heterdimer containing NCKAP1 and CYFIP1 interacts with a heterotrimer formed by WAVE1, ABI2 and BRK1. CYFIP2 binds to activated RAC1 which causes the complex to dissociate, releasing activated WASF1. The complex can also be activated by NCK1 (By similarity). Interacts with ABL1 and ABL2. Subcellular Location: Cytoplasm (By similarity). Isoform 1: Cell projection, lamellipodium (By similarity). Cell projection, filopodium (By similarity). Cytoplasm, cytoskeleton (By similarity). Note=Isoform 1 but not isoform 3 is localized to protruding lamellipodia and filopodia tips (By similarity). Post-translational modifications: Is a substrate for ABL1. Similarity: Belongs to the ABI family. Contains 1 SH3 domain. Contains 1 t-SNARE coiled-coil homology domain. SWISS: Q9NYB9 Gene ID: 10152 Database links: Entrez Gene: 10152 Human Entrez Gene: 329165 Mouse Omim: 606442 Human SwissProt: Q9NYB9 Human SwissProt: P62484 Mouse Unigene: 471156 Human Unigene: 212066 Mouse Unigene: 465571 Mouse |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ABI2) Polyclonal Antibody, Unconjugated (bs-5984R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ABI2) Polyclonal Antibody, Unconjugated (bs-5984R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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