| 產(chǎn)品編號(hào) | bs-1215R |
| 英文名稱 | TPH Rabbit pAb |
| 中文名稱 | 色氨酸羥化酶抗體 |
| 別 名 | MGC119994; TPH 1; TPH; TPH1; TPRH; Tryptophan 5 hydroxylase 1; Tryptophan 5 monooxygenase 1; Tryptophan 5 monooxygenase; Tryptophan hydroxylase 1; Tryptophan 5-hydroxylase 1; TPH1_HUMAN. |
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Specific References (5) | bs-1215R has been referenced in 5 publications.
[IF=5.561] Lei Wu. et al. Ethanol Extract of Mao Jian Green Tea Attenuates Gastrointestinal Symptoms in a Rat Model of Irritable Bowel Syndrome with Constipation via the 5-hydroxytryptamine Signaling Pathway. FOODS. 2023 Jan;12(5):1101 WB ; Rat.
[IF=4.996] Baba, Kazuhiko. et al. Upregulation of the hypothalamo-neurohypophysial system and activation of vasopressin neurones attenuates hyperalgesia in a neuropathic pain model rat. SCI REP-UK. 2022 Jul;12(1):1-15 IHC ; Rat.
[IF=3.158] Ryo Kajihara. et al. Water intake releases serotonin from enterochromaffin cells in rat jejunal villi. 2021 Apr 28 IHC ; Rat.
[IF=2.75] Dong, Xian-Zhe, et al. "A representative prescription for emotional disease, Ding-Zhi-Xiao-Wan restores 5-HT system deficit through interfering the synthesis and transshipment in chronic mild stress-induced depressive Rats." Journal of Ethnopharmacology (2013). WB ; Rat.
[IF=1.966] Tian L et al. Gingerol inhibits cisplatin-induced acute and delayed emesis in rats and minks by regulating the central and peripheral 5-HT, SP, and DA systems. J Nat Med. 2019 Nov 25. IHC-P ; rat&mink.
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| 研究領(lǐng)域 | 細(xì)胞生物 神經(jīng)生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號(hào) | |
| 交叉反應(yīng) | Mouse,Rat (predicted: Human,Rabbit,Chicken,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 49 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TPH: 31-150/444 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a member of the aromatic amino acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. Mutations in this gene have been associated with an elevated risk for a variety of diseases and disorders, including schizophrenia, somatic anxiety, anger-related traits, bipolar disorder, suicidal behavior, addictions, and others.[provided by RefSeq, Apr 2009]. Subunit: Homotetramer. Tissue Specificity: Isoform 2 seems to be less widely expressed than isoform 1. Similarity: Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. Contains 1 ACT domain. SWISS: P17752 Gene ID: 7166 Database links: Entrez Gene: 7166 Human Entrez Gene: 21990 Mouse Omim: 191060 Human SwissProt: P17752 Human SwissProt: P17532 Mouse Unigene: 591999 Human Unigene: 248684 Mouse Unigene: 94788 Rat 色氨酸在腦中首先由色氨酸羥化酶(tryoptophan hydroxylase)催化生成5羥色氨酸(5hydroxytryptophan)，再經(jīng)脫羧酶作用生成5羥色胺，色氨酸羥化酶（TPH）是5-HT生物合成的限速酶，因此，色氨酸羥化酶的缺少可導(dǎo)致5-羥色胺的含量下降。色氨酸羥化酶主要在肝臟內(nèi)代謝。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Cerebellum tissue lysates
Lane 3: Mouse Liver tissue lysates
Lane 4: Rat Cerebrum tissue lysates
Lane 5: Rat Cerebellum tissue lysates
Primary: Anti-TPH (bs-1215R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TPH) Polyclonal Antibody, Unconjugated (bs-1215R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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