| 產(chǎn)品編號 | bs-0026R |
| 英文名稱 | Osteopontin Rabbit pAb |
| 中文名稱 | 骨橋蛋白/分泌型磷蛋白1抗體 |
| 別 名 | BNSP; Bone sialoprotein 1; BSP I; BSPI; Early T lymphocyte activation 1; ETA 1; ETA1; MGC110940; Nephropontin; OPN; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; OSTP_HUMAN; secreted phosphoprotein 1(osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); Secreted phosphoprotein 1; SPP 1; SPP-1; SPP1; SPP1/CALPHA1 fusion; Urinary stone protein; Uropontin. OSTP_Mouse. |
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Specific References (28) | bs-0026R has been referenced in 28 publications.
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| 研究領(lǐng)域 | 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Human,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:500-2000,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 34 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse OPN: 221-280/294 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine.
Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. Osteopontin is overexpressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma. Subunit: Ligand for integrin alpha-V/beta-3. Subcellular Location: Secreted. Tissue Specificity: Bone. Found in plasma. Similarity: Belongs to the osteopontin family. SWISS: P10923 Gene ID: 20750 Database links: Entrez Gene: 6696 Human Entrez Gene: 20750 Mouse Omim: 166490 Human SwissProt: P10451 Human SwissProt: P10923 Mouse Unigene: 313 Human 骨橋蛋白（osteopontin）又稱為早期Ｔ淋巴細(xì)胞活化因子Ｉ或分泌型磷蛋白Ｉ,是一種重要的多功能細(xì)胞外基質(zhì)蛋白,在骨組織細(xì)胞、巨噬細(xì)胞、平滑肌細(xì)胞、內(nèi)皮細(xì)胞及上皮細(xì)胞等多種細(xì)胞上表達(dá),在介導(dǎo)細(xì)胞的趨化、聚集、黏附、增殖和遷移,以及骨組織的礦化與重建、免疫調(diào)節(jié)、信號轉(zhuǎn)導(dǎo)和對感染性疾病的免疫能力等方面有著重要的作用。 ＯＰＮ在細(xì)胞免疫中的功能：1.ＯＰＮ對炎性細(xì)胞的作用：炎癥是炎性細(xì)胞(單核巨噬細(xì)胞、淋巴細(xì)胞、中性粒細(xì)胞等)在炎癥部位的聚集,適度的炎癥對機體有益,如抗感染及促進(jìn)組織細(xì)胞功能恢復(fù)等;但過度的炎癥能引起組織損傷等有害作用。ＯＰＮ在炎癥中具有促炎及抗炎雙重作用；2.ＯＰＮ對淋巴細(xì)胞的作用：早期的報道稱ＯＰＮ是Ｔ淋巴細(xì)胞抑制因子,它可抑制Ｔ淋巴細(xì)胞而促進(jìn)Ｂ淋巴細(xì)胞及肥大細(xì)胞的分化、增殖 骨橋蛋白(osteopontin，OPN)是一種含精氨酸—甘氨酸—天冬氨酸(Arg-Gly-Asp，RGD)的分泌型糖基化磷蛋白，已歸類于細(xì)胞外基質(zhì)(extracellular matrix，ECM)蛋白。 OPN既是細(xì)胞因子，又是細(xì)胞黏附分子。 OPN最初是從骨組織分離的，而后在上皮細(xì)胞、胃腸道的分泌物、腎、甲狀腺、睪丸以及一些腫瘤細(xì)胞里都陸續(xù)的發(fā)現(xiàn)了OPN的表達(dá)。在生殖方面，OPN在分泌期子宮內(nèi)膜上皮細(xì)胞、侵襲的滋養(yǎng)細(xì)胞、蛻膜細(xì)胞中表達(dá)。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: HepG2 (Human) Cell Lysate at 30 ug
Lane 2: A549 (Human) Cell Lysate at 30 ug
Lane 3: U251 (Human) Cell Lysate at 30 ug
Primary: Anti-Osteopontin (bs-0026R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 60 kD
Paraformaldehyde-fixed, paraffin embedded (Rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Osteopontin) Polyclonal Antibody, Unconjugated (bs-0026R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: rat shin bone; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-osteopontin Polyclonal Antibody, Unconjugated(bs-0026R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-osteopontin Polyclonal Antibody, Unconjugated(bs-0026R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: HepG2.
Primary Antibody (green line): Rabbit Anti-Osteopontin antibody (bs-0026R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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