| 產品編號 | bsm-62913R |
| 英文名稱 | MUC16 Recombinant Rabbit mAb |
| 中文名稱 | 卵巢癌抗原重組兔單抗 |
| 別 名 | CA125; MUC16; Mucin 16; Mucin16. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 10E12 |
| 交叉反應 | Human |
| 產品應用 | IHC-P=1:50-1:200,IHC-F=1:50-1:200,IF=1:50-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 1519 kDa |
| 檢測分子量 | 1519 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Mucin 16: 12260-12320 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. SWISS: Q8WXI7 Gene ID: 94025 CA125是與卵巢癌細胞相關的膜表面糖蛋白。在正常情況下卵巢細胞中無CA125抗原，但在經期和孕期婦女的卵巢中發(fā)現存在由此種抗原。 該蛋白是上皮性卵巢癌尤其是漿液性腺癌的主要標記，也可用于其他惡性腫瘤的研究，如：乳腺癌、胰腺癌、肝癌和消化系統的腫瘤等。 |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded Human ovarian cancer;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC16 Monoclonal Antibody, Unconjugated (bsm-62913R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Uterus;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC16 Monoclonal Antibody, Unconjugated (bsm-62913R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human endometrial;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC16 Monoclonal Antibody, Unconjugated (bsm-62913R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
(Negative control)Paraformaldehyde-fixed, paraffin embedded Human colon;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with MUC16 Monoclonal Antibody, Unconjugated (bsm-62913R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
