| 產品編號 | bsm-62553R |
| 英文名稱 | ADRA1A Recombinant Rabbit mAb |
| 中文名稱 | alpha 1腎上腺素能受體A重組兔單抗 |
| 別 名 | ADRA1A; Adra1c; ADRA1L1; ALPHA1AAR. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 11C3 |
| 交叉反應 | Human,Mouse,Rat |
| 產品應用 | WB=1:500-1:2000,Flow-Cyt=1:20-1:100,ICC/IF=1:50-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 51 kDa |
| 檢測分子量 | 52 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human ADRA1A: 200-250 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. SWISS: P35348 Gene ID: 148 ADRA1腎上腺素能α1受體位于突觸后，在血管平滑肌上，興奮時可使血管收縮; alpha 1-adrenergic receptor有興奮效應也有抑制效應。腎上腺素能受體又可分為α和β兩種。alpha 受體與兒茶酚胺結合后，主要是興奮平滑肌，如血管收縮、子宮收縮和瞳孔開張肌收縮等；但也有抑制作用，如使小腸平滑肌舒張。β受體又可分為β1和β2兩個亞型. |
| 產品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with ADRA1A monoclonal antibody, unconjugated (bsm-62553R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
