| 產品編號 | bsm-61882R |
| 英文名稱 | ErbB4 Recombinant Rabbit mAb |
| 中文名稱 | HER4重組兔單抗 |
| 別 名 | ERBB4; HER4; kinase ErbB4; p180erbB4. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse,Rat |
| 產品應用 | WB=1:500-2000,Flow-Cyt=1:20-50,ICC/IF=1:50-200,IP=1:20-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 147 kDa |
| 檢測分子量 | 160 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human ErbB4: 1201-1308 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins and EGF family members and regulates development of the heart, the central nervous system and the mammary gland, gene transcription, cell proliferation, differentiation, migration and apoptosis. Required for normal cardiac muscle differentiation during embryonic development, and for postnatal cardiomyocyte proliferation. Required for normal development of the embryonic central nervous system, especially for normal neural crest cell migration and normal axon guidance. SWISS: Q15303 Gene ID: 2066 細胞膜受體（Membrane Receptors) c-erbB-4蛋白過去一直為乳腺癌基因研究的主要方向，研究乳腺癌組織學分級及術后生存期的關系。目前認為他與細胞增殖活性和激素受體有一定的關聯(lián)，有學者將c-erbB-4蛋白研究用于神經內分泌蛋白為主。 |
| 產品圖片 |
Western blot analysis of (1) Human brain lysate; (2) MCF-7 cell lysate. Using ErbB4 (bsm-61882R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
