| 產(chǎn)品編號 | bsm-61749R |
| 英文名稱 | RARB Recombinant Rabbit mAb |
| 中文名稱 | 維甲酸受體β重組兔單抗 |
| 別 名 | HAP; NR1B2; RAR B; RAR beta; RAR epsilon; RARB; RRB2. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 15A16 |
| 交叉反應 | Human |
| 產(chǎn)品應用 | WB=1:500-1:2000,IHC-P=1:50-1:200,IHC-F=1:50-1:200,IF=1:50-1:200,ICC/IF=1:50-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 50 kDa |
| 檢測分子量 | 55 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human Retinoic acid receptor beta: 400-455 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. SWISS: P10826 Gene ID: 5915 RAR-β參與對很多惡性腫瘤細胞的誘導分化和凋亡作用。 |
| 產(chǎn)品圖片 |
Western blot analysis of MCF7 cell lysate. Using RARB (bsm-61749R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed MCF-7 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (RARB) monoclonal Antibody, unconjugated (bsm-61749R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
