| 產(chǎn)品編號 | bsm-61687R |
| 英文名稱 | EGR1 Recombinant Rabbit mAb |
| 中文名稱 | 早期生長應(yīng)答蛋白1重組兔單抗 |
| 別 名 | TIS8; AT225; G0S30; NGFI-A; ZNF225; KROX-24; ZIF-268; EGR1. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 10F6 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 58 kDa |
| 檢測分子量 | 80 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human EGR 1: 50-200 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'(EGR-site) in the promoter region of target genes. Binds double-stranded target DNA, irrespective of the cytosine methylation status. SWISS: P18146 Gene ID: 1958 Egr-1為即刻早期基因家族中的一個成員,是一種含3個鋅指結(jié)構(gòu)的轉(zhuǎn)錄因子。有研究表明EGR-1在細(xì)胞生長分化中發(fā)揮重要作用，如在細(xì)胞周期G1-S期的轉(zhuǎn)換過程中Egr-1被誘導(dǎo)表達(dá)。 |
| 產(chǎn)品圖片 |
Western blot analysis of 293T cell lysate treated with 0.2 FBS. Using EGR1 (bsm-61687R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with EGR1 Monoclonal Antibody, Unconjugated(bsm-61687R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with EGR1 Monoclonal Antibody, Unconjugated(bsm-61687R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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