| 產(chǎn)品編號(hào) | bsm-61465R |
| 英文名稱 | XRCC1 Recombinant Rabbit mAb |
| 中文名稱 | X射線修復(fù)交叉互補(bǔ)蛋白重組兔單抗 |
| 別 名 | DNA repair protein XRCC1; RCC; X ray repair complementing defective repair in chinese hamster; XRCC 1. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號(hào) | 5C16 |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,ICC/IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 69 kDa |
| 檢測(cè)分子量 | 90 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human XRCC1: 1-50/633 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Scaffold protein involved in DNA single-strand break repair by mediating the assembly of DNA break repair protein complexes. Negatively regulates ADP-ribosyltransferase activity of PARP1 during base-excision repair in order to prevent excessive PARP1 activity. SWISS: P18887 Gene ID: 7515 XRCC-1是一種重要的DNA修復(fù)基因,在一系列內(nèi)外氧化劑(包括煙草成分)導(dǎo)致的DNA單鏈斷裂和堿基損傷修復(fù)中起到重要作用,是目前腫瘤研究的重要的蛋白。 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with XRCC1 monoclonal antibody, unconjugated (bsm-61465R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed K562 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (XRCC1) monoclonal Antibody, unconjugated (bsm-61465R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
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