| 產(chǎn)品編號(hào) | bsm-61284R |
| 英文名稱 | Smac Recombinant Rabbit mAb |
| 中文名稱 | 線粒體促凋亡蛋白重組兔單抗 |
| 別 名 | SMAC; DFNA64; DIABLO; SMAC3. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號(hào) | 8E4 |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,Flow-Cyt=1:20-50,ICC/IF=1:50-200,IP=1:20-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 27 kDa |
| 檢測(cè)分子量 | 21 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human DIABLO: 200-239/239 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. SWISS: Q9NR28 Gene ID: 56616 Smac（second mitochondria-derived activator of caspase）又稱DIABLO（direct IAP binding protein），是一種線粒體蛋白，也是重要的促細(xì)胞凋亡蛋白之一，能夠促進(jìn)細(xì)胞凋亡，當(dāng)殺死不正常細(xì)胞的機(jī)制啟動(dòng)后，線粒體釋放出Smac蛋白促使受損或不正常細(xì)胞凋亡的生化過程就會(huì)出現(xiàn)，Smac在誘導(dǎo)腫瘤細(xì)胞凋亡，增加腫瘤細(xì)胞的化療敏感性及增強(qiáng)免疫力等方面有一定的意義和應(yīng)用前景。Smac是第二個(gè)線粒體來源的胱氨酸酶激活劑，它通過拮抗或中和一種或幾種 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with Smac monoclonal antibody, unconjugated (bsm-61284R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Smac Monoclonal Antibody, Unconjugated(bsm-61284R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Smac Monoclonal Antibody, Unconjugated(bsm-61284R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Smac Monoclonal Antibody, Unconjugated(bsm-61284R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Smac Monoclonal Antibody, Unconjugated(bsm-61284R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Smac Monoclonal Antibody, Unconjugated(bsm-61284R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Smac Monoclonal Antibody, Unconjugated(bsm-61284R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Smac Monoclonal Antibody, Unconjugated(bsm-61284R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
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